| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Replication protein A 70 kDa DNA-binding subunit;RP-A p70;Replication factor A protein 1;RF-A protein 1;Single-stranded DNA-binding protein |
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Product Overview
U-Blot® RPA70 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against RPA70. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Rat.
Validated Applications
| Target | RPA70 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1542R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 68 kDa | Observed: 68 kDa |
| UniProt / Swiss-Prot | P27694 (Human) | Q8VEE4 (Mouse) |
Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Replication protein A 70 kDa DNA-binding subunit
Reactivity Notes
Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.
Scientific Background
function:It participates in a very early step in initiation. RP-A is a single-stranded DNA-binding protein. Absolutely required for simian virus 40 DNA replication in vitro.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the replication factor A protein 1 family.,subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Interacts with RIP and XPA. Interacts with RPA4.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1542R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
