| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized phosho peptide around human Rpb1 CTD (Thr4) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Rpb1 CTD (Phospho Thr4) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Rpb1. Recognizes Phospho. It has been validated for Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Rpb1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human Mouse Rat POLR2A carboxy-terminal domain (CTD) heptapeptide repeat YSPTSPS (phospho-Thr4) |
| Molecular Weight | Observed: 250 kDa |
| UniProt / Swiss-Prot | P24928 (Human) | P08775 (Mouse) |
Recommended dilutions: WB 1:1000-2000
Immunogen / Antigen
Synthesized phosho peptide around human Rpb1 CTD (Thr4)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000-2000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
