| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | POLR2A;POLR2;DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1 |
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| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Rpb1 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Rpb1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA, ChIP and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Rpb1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0570R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA, ChIP |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 192 kDa | Observed: 250 kDa |
| UniProt / Swiss-Prot | P24928 (Human) | P08775 (Mouse) |
Recommended dilutions: IHC 1:50-1:200;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;CHIP 1:50-1:100;Cut&Tag 1:50-1:100
Immunogen / Antigen
DNA-directed RNA polymerase II subunit RPB1
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA, ChIP. Recommended dilutions: IHC 1:50-1:200;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200;CHIP 1:50-1:100;Cut&Tag 1:50-1:100
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0570R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
