U-Blot® RPGF2 Rabbit Polyclonal Antibody

SKU:BHA21810587
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-RPGF2 antibody (Rabbit host, polyclonal, Unconjugated). Validated for Western Blot, ELISA with reactivity to Human, Rat, Mouse.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Rat, Mouse
Conjugate Unconjugated
Isotype IgG
Applications Western Blot, ELISA
Options selector
Catalog no. Size
WP1568-50 50 uL
WP1568-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target RPGF2
Antigen
  • Rap guanine nucleotide exchange factor 2 (Neural RAP guanine nucleotide exchange protein) (nRap GEP) (PDZ domain-containing guanine nucleotide exchange factor 1) (PDZ-GEF1) (RA-GEF)
Gene ID 9693 (Human)
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Rat
  • Mouse
Applications
  • Western Blot
  • ELISA
Immunogen Synthesized peptide derived from human protein . at AA range: 190-270
Conjugate
  • Unconjugated
Molecular weight Observed: 164 kDa
Purification Affinity Purified
Cellular localization Cytoplasm. Cytoplasm, perinuclear region. Cell membrane. Late endosome. Cell junction . Associated with the synaptic plasma membrane. Colocalizes with ADRB1 at the plasma membrane. Synaptosome. Enriched in synaptic plasma membrane and neuronal cell body. Colocalized with CTNNB1 at cell-cell contacts (By similarity). Localized diffusely in the cytoplasm before neuronal growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with the high affinity nerve growth factor receptor NTRK1 at late endosomes. Translocated to the perinuclear region in a RAP1A-dependent manner. Translocated to the cell membrane. .
Concentration 1 mg/ml
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WP1568
Main SKU BHA21810587
Antibody

Product Overview

U-Blot® RPGF2 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RPGF2. It has been validated for Western Blot, ELISA and reacts with Human, Rat, Mouse.

Validated Applications

Target RPGF2
Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Rat, Mouse
Applications Western Blot, ELISA
Purification Affinity Purified
Specificity RPGF2 Polyclonal Antibody detects endogenous levels of protein.
Molecular Weight Observed: 164 kDa
UniProt / Swiss-Prot Q9Y4G8 (Human) | Q8CHG7 (Mouse) | F1M386 (Rat)

Recommended dilutions: WB 1:500-2000;ELISA 1:5000-20000

Immunogen / Antigen

Synthesized peptide derived from human protein . at AA range: 190-270

Reactivity Notes

Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.

Scientific Background

Members of the RAS (see HRAS; MIM 190020) subfamily of GTPases function in signal transduction as GTP/GDP-regulated switches that cycle between inactive GDP- and active GTP-bound states. Guanine nucleotide exchange factors (GEFs), such as RAPGEF2, serve as RAS activators by promoting acquisition of GTP to maintain the active GTP-bound state and are the key link between cell surface receptors and RAS activation (Rebhun et al., 2000 [PubMed 10934204]).[supplied by OMIM, Mar 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;ELISA 1:5000-20000

What species does it react with?

Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

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