| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Run domain Beclin-1 interacting and cystein-rich containing protein;Rubicon;Beclin-1 associated RUN domain containing protein;Baron |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human RUBIC AA range: 868-918 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® RUBIC Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RUBIC. It has been validated for Western Blot and reacts with Human, Mouse.
Validated Applications
| Target | RUBIC |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of RUBIC at Human/Mouse |
| Molecular Weight | Observed: 105 kDa |
| UniProt / Swiss-Prot | Q92622 (Human) | Q80U62 (Mouse) |
Recommended dilutions: WB 1:500-2000
Immunogen / Antigen
Synthesized peptide derived from human RUBIC AA range: 868-918
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a negative regulator of autophagy and endocytic trafficking and controls endosome maturation. This protein contains two conserved domains, an N-terminal RUN domain and a C-terminal DUF4206 domain. The RUN domain is involved in Ras-like GTPase signaling, and the DUF4206 domain contains a diacylglycerol (DAG) binding-like motif. Mutation in this gene results in deletion of the DAG binding-like motif and causes a recessive ataxia. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Apr 2014]
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:500-2000
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
