| Field | Specification |
|---|---|
| Alternative Names | RUNX2;AML3;CBFA1;OSF2;PEBP2A;Runt-related transcription factor 2;Acute myeloid leukemia 3 protein;Core-binding factor subunit alpha-1;CBF-alpha-1;Oncogene AML-3Osteoblast-specific transcription factor 2;OSF-2;Polyomavirus enhancer-binding protein 2 alpha A subunit;PEA2-alpha A;PEBP2-alpha A;SL3-3 enhancer factor 1 alpha A subunit;SL3/AKV core-binding factor alpha A subunit |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the Internal region of human RUNX2. AA range:201-250 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® RUNX2 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against RUNX2. It has been validated for Immunofluorescence, Western Blot, ELISA and reacts with Human, Mouse, Rat, Dog.
Validated Applications
| Target | RUNX2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Dog |
| Applications | Immunofluorescence, Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | RUNX2 Polyclonal Antibody detects endogenous levels of RUNX2 protein. |
| Molecular Weight | Observed: 56 kDa |
| UniProt / Swiss-Prot | Q13950 (Human) | Q08775 (Mouse) | Q9Z2J9 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from the Internal region of human RUNX2. AA range:201-250
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Dog. Confirm suitability for untested species before use.
Scientific Background
This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing. [provided by RefSeq, Jul 2016]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;ELISA 1:20000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse, Rat, Dog. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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