| Field | Specification |
|---|---|
| Alternative Names | JNK2, PRKM9, SAPK1A |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | polypeptide |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® SAPK/JNK (phospho T183/Y185) Mouse mAb is a recombinant monoclonal antibody raised in Mouse that specifically recognizes Phospho-SAPK/JNK (Thr183/Tyr185) (Phosphorylation [Thr183/Tyr185]). It is designed for detection and analysis of the Phosphorylation [Thr183/Tyr185] post-translational modification across research applications.
The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists. As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase. Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs. Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member. There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms. SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors.
Validated Applications
| Application | Western Blot |
|---|---|
| Recommended Dilution | WB 1:1000 |
| Host | Mouse |
| Clonality | Recombinant Monoclonal |
| Isotype | IgG1 |
| Reactivity | Human |
| Conjugate | Unconjugated |
| Purification | Protein A |
| Molecular Weight | Calculated: 48 kDa | Observed: 46, 54 kDa |
Immunogen / Antigen
polypeptide
Reactivity Notes
This antibody shows reactivity with Human. It targets the Phosphorylation [Thr183/Tyr185] modification and is suitable for Western Blot.
Safety & Handling
Related Products
Explore other UcallM Biosciences post-translational modification antibodies available through BioHippo.
It is validated for Western Blot. Recommended dilutions: WB 1:1000.
Use WB 1:1000. Optimal dilution should be determined empirically for your sample and detection system.
This is a pan-modification antibody recognizing Phospho-SAPK/JNK (Thr183/Tyr185) (Phosphorylation [Thr183/Tyr185]); reactivity: Human.
The recombinant monoclonal antibody (Mouse host) was raised against polypeptide.
This product is supplied unconjugated. Contact BioHippo for custom conjugation or format options.
Store at -20°C. Avoid repeated freeze-thaw cycles | Stable for 12 months from date of receipt / reconstitution.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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