| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | SHC-transforming protein 1;SHC-transforming protein 3;SHC-transforming protein A;Src homology 2 domain-containing-transforming protein C1;SH2 domain protein C1 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human SHC1 (Phospho Tyr349+Tyr350) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® SHC1 (Phospho Tyr349+Tyr350) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Shc. Recognizes Phospho. It has been validated for Immunohistochemistry, Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Shc |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of SHC1 (Phospho Tyr349+Tyr350) Rabbit pAb at Human, Mouse,Rat |
| Molecular Weight | Observed: 66 kDa(p66),52 kDa(p52),46 kDa(p46)kD |
| UniProt / Swiss-Prot | P29353 (Human) | P98083 (Mouse) | Q5M824 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-200
Immunogen / Antigen
Synthesized peptide derived from human SHC1 (Phospho Tyr349+Tyr350)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
SHC adaptor protein 1(SHC1) Homo sapiens This gene encodes three main isoforms that differ in activities and subcellular location. While all three are adapter proteins in signal transduction pathways, the longest (p66Shc) may be involved in regulating life span and the effects of reactive oxygen species. The other two isoforms, p52Shc and p46Shc, link activated receptor tyrosine kinases to the Ras pathway by recruitment of the GRB2/SOS complex. p66Shc is not involved in Ras activation. Unlike the other two isoforms, p46Shc is targeted to the mitochondrial matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011]
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot. Recommended dilutions: WB 1:500-2000;IHC 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
