| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | SIRT1;SIR2L1;NAD-dependent protein deacetylase sirtuin-1;hSIRT1;Regulatory protein SIR2 homolog 1;SIR2-like protein 1;hSIR2 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® SIRT1 Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against SIRT1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Rat.
Validated Applications
| Target | SIRT1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0158R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | Endogenous |
| Molecular Weight | Calculated: 81 kDa | Observed: 130 kDa |
| UniProt / Swiss-Prot | Q96EB6 (Human) | Q923E4 (Mouse) |
Recommended dilutions: IHC 1:100-200;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000;IP 1:50-200
Immunogen / Antigen
NAD-dependent protein deacetylase sirtuin-1
Reactivity Notes
Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: IHC 1:100-200;WB 1:1000-5000;IF 1:200-1000;ELISA 1:5000-20000;IP 1:50-200
Confirmed reactivity: Human, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0158R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
