| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Lymphocyte cytosolic protein 2;SH2 domain-containing leukocyte protein of 76 kDa;SLP-76 tyrosine phosphoprotein;SLP76 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized phosho peptide around human SLP-76 (Tyr145) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® SLP-76 (Phospho Tyr145) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against SLP-76. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry and reacts with Human, Mouse.
Validated Applications
| Target | SLP-76 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human Mouse SLP-76 (phospho-Tyr145) |
| Molecular Weight | Observed: 75 kDa |
| UniProt / Swiss-Prot | Q13094 (Human) | Q60787 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Immunogen / Antigen
Synthesized phosho peptide around human SLP-76 (Tyr145)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
SLP-76 was originally identified as a substrate of the ZAP-70 protein tyrosine kinase following T cell receptor (TCR) ligation in the leukemic T cell line Jurkat. The SLP-76 locus has been localized to human chromosome 5q33 and the gene structure has been partially characterized in mice. The human and murine cDNAs both encode 533 amino acid proteins that are 72% identical and comprised of three modular domains. The NH2-terminus contains an acidic region that includes a PEST domain and several tyrosine residues which are phosphorylated following TCR ligation. SLP-76 also contains a central proline-rich domain and a COOH-terminal SH2 domain. A number of additional proteins have been identified that associate with SLP-76 both constitutively and inducibly following receptor ligation, supporting the notion that SLP-76 functions as an adaptor or scaffold protein. Studies using SLP-76 deficient T c
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
