| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | SMAD2;MADH2;MADR2;Mothers against decapentaplegic homolog 2;MAD homolog 2;Mothers against DPP homolog 2;JV18-1;Mad-related protein 2;hMAD-2;SMAD family member 2;SMAD 2;Smad2;hSMAD2;SMAD3;MADH3;Mothers against decapentaplegic |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8. AA range:1-50 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Smad2/3 (Phospho Thr8) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Smad2/3. Recognizes Phospho. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat, Pig.
Validated Applications
| Target | Smad2/3 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Pig |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Smad2/3 (T8) Polyclonal Antibody detects endogenous levels of Smad2/3 protein only when phosphorylated at T8. |
| Molecular Weight | Observed: 48 kDa |
| UniProt / Swiss-Prot | Q15796;P84022 (Human) | O70436;P84025 (Rat) |
Recommended dilutions: WB 1:500-2000;IF/ICC1:100-500;ELISA 1:5000-20000
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Smad2/3 around the phosphorylation site of Thr8. AA range:1-50
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Pig. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IF/ICC1:100-500;ELISA 1:5000-20000
Confirmed reactivity: Human, Mouse, Rat, Pig. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
