| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | SMAD3 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of Smad3 at AA range of 350-430 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Smad3 Mouse Monoclonal Antibody (4C9) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Smad3. It has been validated for Western Blot, Immunofluorescence, ELISA and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Smad3 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4C9 |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Smad3 protein detects endogenous levels of SMAD3 |
| Molecular Weight | Observed: 52 kDa |
| UniProt / Swiss-Prot | P84022 (Human) | Q8BUN5 (Mouse) | P84025 (Rat) |
Recommended dilutions: WB 1:1000-2000;IF 1:50-200
Immunogen / Antigen
Synthetic Peptide of Smad3 at AA range of 350-430
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, ELISA. Recommended dilutions: WB 1:1000-2000;IF 1:50-200
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 4C9.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
