| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | SMAD5;MADH5;Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5 |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human Smad5 (C-terminus) protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Smad5 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Smad5. It has been validated for Western Blot, Immunofluorescence, Flow Cytometry and reacts with Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep.
Validated Applications
| Target | Smad5 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep |
| Applications | Western Blot, Immunofluorescence, Flow Cytometry |
| Purification | Affinity Purified |
| Specificity | Smad5 Monoclonal Antibody detects endogenous levels of Smad5 protein. |
| Molecular Weight | Calculated: 52 kDa |
| UniProt / Swiss-Prot | Q99717 (Human) | P97454 (Mouse) | Q9R1V3 (Rat) |
Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Flow Cyt 1:100-1:200;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human Smad5 (C-terminus) protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Flow Cytometry. Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Flow Cyt 1:100-1:200;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
