| Field | Specification |
|---|---|
| Alternative Names | SMAD5;MADH5;Mothers against decapentaplegic homolog 5;MAD homolog 5;Mothers against DPP homolog 5;JV5-1;SMAD family member 5;SMAD 5;Smad5;hSmad5 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from the Internal region of human Smad5. |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Smad5 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Smad5. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Rat.
Validated Applications
| Target | Smad5 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | Smad5 Polyclonal Antibody detects endogenous levels of Smad5 protein. |
| Molecular Weight | Observed: 52 kDa |
| UniProt / Swiss-Prot | Q99717 (Human) | P97454 (Mouse) | Q9R1V3 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:40000;IF 1:50-200
Immunogen / Antigen
Synthesized peptide derived from the Internal region of human Smad5.
Reactivity Notes
Confirmed cross-reactivity: Human, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:40000;IF 1:50-200
Confirmed reactivity: Human, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
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