| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Sodium/potassium-transporting ATPase subunit alpha-1;Na;+;/K;+;ATPase alpha-1 subunit;Sodium pump subunit alpha-1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide from human protein at AA range: 230-290 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Sodium Potassium ATPase alpha-1 (Phospho Tyr260) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Na+/K+-ATPase α1. Recognizes Phospho. It has been validated for Western Blot, ELISA, Immunohistochemistry and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Na+/K+-ATPase α1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot, ELISA, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | The antibody detects endogenous Sodium Potassium ATPase alpha-1 when Phospho occurs at Tyr260) |
| Molecular Weight | Observed: 115 kDa |
| UniProt / Swiss-Prot | P05023 (Human) | Q8VDN2 (Mouse) | P06685 (Rat) |
Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Immunogen / Antigen
Synthetic peptide from human protein at AA range: 230-290
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
Safety & Handling
This antibody is validated for Western Blot, ELISA, Immunohistochemistry. Recommended dilutions: WB 1:500-2000;IHC 1:50-300;ELISA 1:2000-20000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
