| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Sphingosine kinase 1;SK 1;SPK 1 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from human SPHK1 (Phospho-Ser225) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® SPHK1 (Phospho Ser225) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against SPHK1. Recognizes Phospho. It has been validated for Western Blot and reacts with Human, Mouse, Rat.
Validated Applications
| Target | SPHK1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of SPHK1 (Phospho-Ser225) at Human, Mouse,Rat |
| Molecular Weight | Observed: 42 kDa |
| UniProt / Swiss-Prot | Q9NYA1 (Human) | Q8CI15 (Mouse) | Q91V26 (Rat) |
Recommended dilutions: WB 1:500-2000
Immunogen / Antigen
Synthesized peptide derived from human SPHK1 (Phospho-Ser225)
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:500-2000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
