| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | SPHK2;Sphingosine kinase 2;SK 2;SPK 2 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human SPHK2 around the phosphorylation site of Thr614. AA range:580-629 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® SphK2 (Phospho Thr614) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against SphK2. Recognizes Phospho. It has been validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | SphK2 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Immunofluorescence, Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-SphK2 (T614) Polyclonal Antibody detects endogenous levels of SphK2 protein only when phosphorylated at T614. |
| Molecular Weight | Calculated: 69 kDa |
| UniProt / Swiss-Prot | Q9NRA0 (Human) | Q9JIA7 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human SPHK2 around the phosphorylation site of Thr614. AA range:580-629
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes one of two sphingosine kinase isozymes that catalyze the phosphorylation of sphingosine into sphingosine 1-phosphate. Sphingosine 1-phosphate mediates many cellular processes including migration, proliferation and apoptosis, and also plays a role in several types of cancer by promoting angiogenesis and tumorigenesis. The encoded protein may play a role in breast cancer proliferation and chemoresistance. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
Safety & Handling
This antibody is validated for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;ELISA 1:5000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
