| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | STAT4;Signal transducer and activator of transcription 4 |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT4 around the phosphorylation site of Tyr693. AA range:660-709 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Stat4 (Phospho Tyr693) Rabbit Polyclonal Antibody (WP9285) is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Stat4. Recognizes Phospho. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | Stat4 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-Stat4 (Y693) Polyclonal Antibody detects endogenous levels of Stat4 protein only when phosphorylated at Y693. |
| Molecular Weight | Observed: 86 kDa |
| UniProt / Swiss-Prot | Q14765 (Human) | P42228 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;ELISA 1:5000;IF 1:50-200
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human STAT4 around the phosphorylation site of Tyr693. AA range:660-709
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC 1:100-1:300;IP 2-5 ug:mg lysate;ELISA 1:5000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
