| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | STAT5A;STAT5;Signal transducer and activator of transcription 5A;STAT5B;Signal transducer and activator of transcription 5B |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Isotype | |
| Molecular Weight | |
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| Target |
Product Overview
U-Blot® STAT5 (Phospho Tyr694) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against STAT5A/B. Recognizes Phospho (Tyr694). It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | STAT5A/B |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0067R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Protein A |
| Specificity | This antibody detects endogenous levels of STAT5A/B only when phosphorylated at Tyr694.This antibody does not recognize phosphorylated at other sites. |
| Molecular Weight | Calculated: 91 kDa | Observed: 105 kDa |
| UniProt / Swiss-Prot | P42229;P51692 (Human) | Q62771;P52632 (Rat) |
Recommended dilutions: IHC 1:100-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Immunogen / Antigen
Signal transducer and activator of transcription 5A/B
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:100-1:500;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0067R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
