| Field | Specification |
|---|---|
| Target | |
| Alternative names | KIAA0640;70kDa;AV235546;FLJ39540;HSPC321;SWA 70 protein;SWAP 70;SWAP switching B-cell complex 70kDa subunit;SWAP-70;SWAP70;Switch associated protein 70;Switch-associated protein 70;SWP70_HUMAN. |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human SWAP70 protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® SWAP70 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against SWAP70. It has been validated for Western Blot, Immunoprecipitation and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | SWAP70 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Western Blot, Immunoprecipitation |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of SWAP70 and does not cross-react with related proteins. |
| Molecular Weight | Observed: 70 kDa |
| UniProt / Swiss-Prot | Q9UH65 (Human) | Q6A028 (Mouse) |
Recommended dilutions: WB 1:1000
Immunogen / Antigen
Purified recombinant human SWAP70 protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
domain:The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding.,function:Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.,PTM:Tyrosine-phosphorylated.,similarity:Contains 1 PH domain.,subcellular location:In resting B-cells it is localized mainly in the cytoplasm and upon cell activation it is recruited to the plasma membrane and then translocates to the nucleus. In activated, class-switching B-cells it is associated with membrane IgG but not IgM. Localized to loose actin filament arrays located behind actively extending lamellipodia.,subunit:The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70.,tissue specificity:Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760).
Safety & Handling
This antibody is validated for Western Blot, Immunoprecipitation. Recommended dilutions: WB 1:1000
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
