| Field | Specification |
|---|---|
| Target | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Protein of TAP Tag |
| Conjugate | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® TAP-Tag Mouse Monoclonal Antibody (Mix) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against TAP Tag. It has been validated for Western Blot and reacts with the listed species.
Validated Applications
| Target | TAP Tag |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Species independent |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | The antibody detects TAP recombinant protein. |
Recommended dilutions: WB 1:500-10000
Immunogen / Antigen
Recombinant Protein of TAP Tag
Scientific Background
The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB), a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags. The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes.
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:500-10000
Confirmed reactivity: the listed species. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
