| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | GTF2B;TF2B;TFIIB;Transcription initiation factor IIB;General transcription factor TFIIB;S300-II |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human TFIIB protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® TFIIB Mouse Monoclonal Antibody (WM0543) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against TFIIB. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence and reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Pig.
Validated Applications
| Target | TFIIB |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Bovine, Chicken, Dog, Pig |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | TFIIB Monoclonal Antibody detects endogenous levels of TFIIB protein. |
| Molecular Weight | Calculated: 35 kDa |
| UniProt / Swiss-Prot | Q00403 (Human) | P62915 (Mouse) | P62916 (Rat) |
Recommended dilutions: WB 1:1000-1:2000;IHC 1:500-1:1000;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human TFIIB protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Pig. Confirm suitability for untested species before use.
Scientific Background
general transcription factor IIB(GTF2B) Homo sapiens This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;IHC 1:500-1:1000;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Bovine, Chicken, Dog, Pig. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
