U-Blot® TFIIB Mouse Monoclonal Antibody (WM0557)

SKU:BHA21802136
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-TFIIB antibody (Mouse host, monoclonal, Unconjugated). Validated for Western Blot with reactivity to Human, Mouse, Monkey.
Clonality Monoclonal
Host Mouse
Reactivity Human, Mouse, Monkey
Conjugate Unconjugated
Applications Western Blot
Options selector
Catalog no. Size
WM0557-50 50 uL
WM0557-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target TFIIB
Alternative names General transcription factor IIB;General transcription factor TFIIB;gtf2b;RNA polymerase II transcription factor IIB;S300 II;S300-II;TF IIB;TF2B;TF2B_HUMAN;TFIIB;Transcription initiation factor IIB.
Gene ID 2959 (Human)
Host Mouse
Clonality
  • Monoclonal
Reactivity
  • Human
  • Mouse
  • Monkey
Applications
  • Western Blot
Immunogen Purified recombinant human TFIIB protein fragments expressed in E.coli.
Conjugate
  • Unconjugated
Molecular weight Observed: 34 kDa
Purification Affinity Purified
Cellular localization Nucleus . Chromosome . Non-acetylated form colocalizes with DNA in the G0/1, S and G2 phases of the cell cycle, but not during mitosis (PubMed:24441171). Acetylated form colocalizes at transcriptionally silent mitotic chromatids during mitosis at metaphase, anaphase, and telophase phases of the cell cycle (PubMed:24441171). .
Formulation Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WM0557
Main SKU BHA21802136
Antibody

Product Overview

U-Blot® TFIIB Mouse Monoclonal Antibody (WM0557) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against TFIIB. It has been validated for Western Blot and reacts with Human, Mouse, Monkey.

Validated Applications

Target TFIIB
Host Mouse
Clonality Monoclonal
Conjugate Unconjugated
Reactivity Human, Mouse, Monkey
Applications Western Blot
Purification Affinity Purified
Specificity This antibody detects endogenous levels of TFIIB and does not cross-react with related proteins.
Molecular Weight Observed: 34 kDa
UniProt / Swiss-Prot Q00403 (Human) | P62915 (Mouse)

Recommended dilutions: WB 1:500

Immunogen / Antigen

Purified recombinant human TFIIB protein fragments expressed in E.coli.

Reactivity Notes

Confirmed cross-reactivity: Human, Mouse, Monkey. Confirm suitability for untested species before use.

Scientific Background

general transcription factor IIB(GTF2B) Homo sapiens This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot. Recommended dilutions: WB 1:500

What species does it react with?

Confirmed reactivity: Human, Mouse, Monkey. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.

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