| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | TOP3A;TOP3;DNA topoisomerase 3-alpha;DNA topoisomerase III alpha |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from Topo IIIα . at AA range: 350-430 |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Topo IIIα Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Topo IIIα. It has been validated for Western Blot, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | Topo IIIα |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, ELISA |
| Purification | Affinity Purified |
| Specificity | Topo IIIα Polyclonal Antibody detects endogenous levels of Topo IIIα protein. |
| Molecular Weight | Observed: 115 kDa |
| UniProt / Swiss-Prot | Q13472 (Human) | O70157 (Mouse) |
Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
Synthesized peptide derived from Topo IIIα . at AA range: 350-430
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. This enzyme forms a complex with BLM which functions in the regulation of recombination in somatic cells. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016]
Safety & Handling
This antibody is validated for Western Blot, ELISA. Recommended dilutions: WB 1:500-1:2000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
