| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | DNA topoisomerase 2-alpha;DNA topoisomerase II, alpha isozyme |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized phosho peptide around human Topoisomerase IIα (Ser1469) |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Topoisomerase IIα (Phospho Ser1469) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against Topo IIα. Recognizes Phospho. It has been validated for Western Blot and reacts with Human, Rat, Mouse.
Validated Applications
| Target | Topo IIα |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | This antibody detects endogenous levels of Human Topoisomerase IIα (phospho-Ser1469) |
| Molecular Weight | Observed: 174 kDa |
| UniProt / Swiss-Prot | P11388 (Human) | Q01320 (Mouse) | P41516 (Rat) |
Recommended dilutions: WB 1:1000-2000
Immunogen / Antigen
Synthesized phosho peptide around human Topoisomerase IIα (Ser1469)
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000-2000
Confirmed reactivity: Human, Rat, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
