| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | UBE2A;RAD6A;Ubiquitin-conjugating enzyme E2 A;RAD6 homolog A;HR6A;hHR6A;Ubiquitin carrier protein A;Ubiquitin-protein ligase A;UBE2B;RAD6B;Ubiquitin-conjugating enzyme E2 B;RAD6 homolog B;HR6B;hHR6B;Ubiquitin carrier protein B;Ubiquitin-conjugating enzyme E2-17 kDa;Ubiquitin-protein ligase B |
| Gene ID | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthesized peptide derived from the C-terminal region of human UBE2A/B. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® UBE2A/B Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against UBE2A/B. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | UBE2A/B |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | UBE2A/B Polyclonal Antibody detects endogenous levels of UBE2A/B protein. |
| Molecular Weight | Observed: 17 kDa |
| UniProt / Swiss-Prot | P49459;P63146 (Human) | P63149 (Rat) |
Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:10000;IF 1:50-200
Immunogen / Antigen
Synthesized peptide derived from the C-terminal region of human UBE2A/B.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-1:2000;IHC: 1:100-300;ELISA 1:10000;IF 1:50-200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
