| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic Peptide of Ubiquitin |
| Conjugate | |
| Purification | |
| Concentration | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® Ubiquitin Mouse Monoclonal Antibody (5F1) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Ubiquitin. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry and reacts with Human, Rat, Mouse, Pig.
Validated Applications
| Target | Ubiquitin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5F1 |
| Conjugate | Unconjugated |
| Reactivity | Human, Rat, Mouse, Pig |
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry |
| Purification | Affinity Purified |
| Specificity | Ubiquitin protein detects endogenous levels of Ubiquitin |
| UniProt / Swiss-Prot | P62979;P62987;P62988 (Human) |
Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:200
Immunogen / Antigen
Synthetic Peptide of Ubiquitin
Reactivity Notes
Confirmed cross-reactivity: Human, Rat, Mouse, Pig. Confirm suitability for untested species before use.
Scientific Background
UBB (ubiquitin B) encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. UBB consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of UBB are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants.
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry. Recommended dilutions: WB 1:1000-2000;IHC 1:100-200;IF 1:200
Confirmed reactivity: Human, Rat, Mouse, Pig. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone 5F1.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
