| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | UBN1;Ubinuclein-1;HIRA-binding protein;Protein VT4;Ubiquitously expressed nuclear protein |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Ubinuclein. AA range:161-210 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® UBN-1 Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against UBN-1. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human, Mouse.
Validated Applications
| Target | UBN-1 |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse |
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence, ELISA |
| Purification | Affinity Purified |
| Specificity | UBN-1 Polyclonal Antibody detects endogenous levels of UBN-1 protein. |
| Molecular Weight | Calculated: 122 kDa |
| UniProt / Swiss-Prot | Q9NPG3 (Human) | Q4G0F8 (Mouse) |
Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human Ubinuclein. AA range:161-210
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse. Confirm suitability for untested species before use.
Scientific Background
function:Acts as a novel regulator of senescence. Involved in the formation of senescence-associated heterochromatin foci (SAHF), which represses expression of proliferation-promoting genes. Binds to proliferation-promoting genes. May be required for replication-independent chromatin assembly.,similarity:Belongs to the ubinuclein family.,subcellular location:Localized as a nuclear speckled-like pattern in proliferating primary fibroblasts. Colocalizes with HIRA, PML and SP100 in PML bodies of senescent cells. Colocalizes with TJP1 and CLDN1. Detected along the upper granular cell layer of epidermis. When overexpressed, accumulates in the nucleus in cells showing defective cytokinesis.,subunit:Component of a complex that includes at least ASF1A, CABIN1, HIRA, histone H3.3 and UBN1. Interacts with Epstein-Barr virus BZLF1. Interacts with HIRA (via WD repeat domain); the interaction is direct. Interacts with ASF1A, CEBPA, TJP1, TJP2 and TJP3.,tissue specificity:Ubiquitous. Also expressed in numerous tumors and cancer cell lines.
Safety & Handling
This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: WB 1:500-2000;IHC 1:100-1:300;IF 1:200-1:1000;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
