| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | UHRF1;ICBP90;NP95;RNF106;E3 ubiquitin-protein ligase UHRF1;Inverted CCAAT box-binding protein of 90 kDa;Nuclear protein 95;Nuclear zinc finger protein Np95;HuNp95;hNp95;RING finger protein 106;Transcription factor ICBP90;Ubiquit |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human UHRF1 (N-terminus) protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® UHRF1 Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against UHRF1. It has been validated for Western Blot, Immunofluorescence and reacts with Human.
Validated Applications
| Target | UHRF1 |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human |
| Applications | Western Blot, Immunofluorescence |
| Purification | Affinity Purified |
| Specificity | UHRF1 Monoclonal Antibody detects endogenous levels of UHRF1 protein. |
| Molecular Weight | Calculated: 90 kDa |
| UniProt / Swiss-Prot | Q96T88 (Human) | Q8VDF2 (Mouse) |
Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human UHRF1 (N-terminus) protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence. Recommended dilutions: WB 1:1000-1:2000;IF 1:100-1:500;Not yet tested in other applications.
Confirmed reactivity: Human. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
