| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | ULK1;KIAA0722;Serine/threonine-protein kinase ULK1;Autophagy-related protein 1 homolog;ATG1;hATG1;Unc-51-like kinase 1 |
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| Isotype | |
| Reactivity | |
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Product Overview
U-Blot® ULK1 (Phospho Ser757) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against ULK1. Recognizes Phospho (Ser757). It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | ULK1 |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT0739R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | The antibody detects endogenous ULK1(Phospho AE13) protein. |
| Molecular Weight | Calculated: 113 kDa | Observed: 150 kDa |
| UniProt / Swiss-Prot | O75385 (Human) | O70405 (Mouse) |
Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Serine/threonine-protein kinase ULK1
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with GABARAP and GABARAPL2.,tissue specificity:Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT0739R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
