| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | CKMT1A;CKMT;CKMT1B;CKMT;Creatine kinase U-type;mitochondrial;Acidic-type mitochondrial creatine kinase;Mia-CK;Ubiquitous mitochondrial creatine kinase;U-MtCK |
| Gene ID | |
| Host | |
| Clonality | |
| Reactivity | |
| Applications | |
| Immunogen | Purified recombinant human uMtCK protein fragments expressed in E.coli. |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® uMtCK Mouse Monoclonal Antibody is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against uMtCK. It has been validated for Western Blot and reacts with Human, Mouse, Rat, Dog, Pig, Rabbit.
Validated Applications
| Target | uMtCK |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Dog, Pig, Rabbit |
| Applications | Western Blot |
| Purification | Affinity Purified |
| Specificity | uMtCK Monoclonal Antibody detects endogenous levels of uMtCK protein. |
| Molecular Weight | Calculated: 47 kDa |
| UniProt / Swiss-Prot | P12532 (Human) | P30275 (Mouse) | P25809 (Rat) |
Recommended dilutions: WB 1:1000-1:2000;Not yet tested in other applications.
Immunogen / Antigen
Purified recombinant human uMtCK protein fragments expressed in E.coli.
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit. Confirm suitability for untested species before use.
Scientific Background
Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mi
Safety & Handling
This antibody is validated for Western Blot. Recommended dilutions: WB 1:1000-1:2000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Dog, Pig, Rabbit. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
