| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | VEGFA;VEGF;Vascular endothelial growth factor A;VEGF-A;Vascular permeability factor;VPF |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human VEGF-A. AA range:110-159 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® VEGF-A Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against VEGF-A. It has been validated for Western Blot, Immunofluorescence, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat, Pig, Rabbit.
Validated Applications
| Target | VEGF-A |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Pig, Rabbit |
| Applications | Western Blot, Immunofluorescence, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | VEGF-A Polyclonal Antibody detects endogenous levels of VEGF-A protein. |
| Molecular Weight | Observed: 21 kDa(monomer),42 kDa(dimer) |
| UniProt / Swiss-Prot | P15692 (Human) | Q00731 (Mouse) | P16612 (Rat) |
Recommended dilutions: WB 1:500-1:2000.IF 1:50-200;IHC 1:100-1:300;ELISA 1:10000;Not yet tested in other applications.
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human VEGF-A. AA range:110-159
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Pig, Rabbit. Confirm suitability for untested species before use.
Scientific Background
This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. This gene is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Elevated levels of this protein are found in patients with POEMS syndrome, also known as Crow-Fukase syndrome. Allelic variants of this gene have been associated with microvascular complications of diabetes 1 (MVCD1) and atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been described. There is also evidence for alternative translation initiation fro
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunohistochemistry, ELISA. Recommended dilutions: WB 1:500-1:2000.IF 1:50-200;IHC 1:100-1:300;ELISA 1:10000;Not yet tested in other applications.
Confirmed reactivity: Human, Mouse, Rat, Pig, Rabbit. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
