U-Blot® Villin Rabbit Monoclonal Antibody

SKU:BHA21801205
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UcallM Biosciences Inc
UcallM Biosciences Inc
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Overview
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Anti-Villin antibody (Rabbit host, monoclonal, Unconjugated). Validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA with reactivity to Human.
Clonality Monoclonal
Host Rabbit
Reactivity Human
Conjugate Unconjugated
Isotype IgG, Kappa
Clone PT1451R
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Options selector
Catalog no. Size
WR5217-50 50 uL
WR5217-100 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 50 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -15°C to -25°C/1 year(Do not lower than -25°C)
  • Shipping: cold-chain shipment (typically with ice packs).
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target Villin
Antigen
  • Villin-1
Alternative names villin 1
Gene ID 7429 (Human) | 22349 (Mouse)
Host Rabbit
Clone PT1451R
Clonality
  • Monoclonal
Isotype
  • IgG
  • Kappa
Reactivity
  • Human
Applications
  • Western Blot
  • Immunohistochemistry
  • Immunofluorescence
  • ELISA
Conjugate
  • Unconjugated
Molecular weight Calculated: 93 kDa | Observed: 93 kDa
Purification Protein A
Cellular localization Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. .
Formulation PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Storage -15°C to -25°C/1 year(Do not lower than -25°C)
Catalog no. (Mfr.) WR5217
Main SKU BHA21801205
Antibody

Product Overview

U-Blot® Villin Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against Villin. It has been validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA and reacts with Human.

Validated Applications

Target Villin
Host Rabbit
Clonality Monoclonal
Clone PT1451R
Isotype IgG, Kappa
Conjugate Unconjugated
Reactivity Human
Applications Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purification Protein A
Specificity Endogenous
Molecular Weight Calculated: 93 kDa | Observed: 93 kDa
UniProt / Swiss-Prot P09327 (Human) | Q62468 (Mouse)

Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000

Immunogen / Antigen

Villin-1

Reactivity Notes

Confirmed cross-reactivity: Human. Confirm suitability for untested species before use.

Scientific Background

This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008]

Safety & Handling

For Research Use Only (RUO). Not for diagnostic or therapeutic use. Store as indicated: -15°C to -25°C/1 year(Do not lower than -25°C)
What applications is this antibody validated for?

This antibody is validated for Western Blot, Immunohistochemistry, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1:1000;WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000

What species does it react with?

Confirmed reactivity: Human. Other species should be validated by the user.

Is this antibody conjugated?

Conjugate: Unconjugated.

How should it be stored?

Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)

What is the clone number?

Clone PT1451R.

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