| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | CTRCT30;Epididymis luminal protein 113;FLJ36605;HEL113;VIM;VIME_HUMAN;Vimentin |
| Cellular Localization | |
| Clonality | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | Synthesized peptide derived from human protein. AA range:400-466 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® Vimentin (ABT019) Mouse Monoclonal Antibody (WM1318) is a mouse monoclonal antibody from UcallM Biosciences Inc, raised against Vimentin. It has been validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | Vimentin |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | ABT019 |
| Isotype | IgG2b, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Immunohistochemistry, Western Blot, Immunofluorescence, ELISA |
| Purification | Protein G |
| Specificity | The antibody can specifically recognize human Vimentin protein. In western blotting of HEK293 cell lysate, the antibody can label a 54 kDa band corresponding to Vimentin. |
| Molecular Weight | Calculated: 53 kDa | Observed: 54 kDa |
| UniProt / Swiss-Prot | P08670 (Human) | P20152 (Mouse) |
Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Immunogen / Antigen
Synthesized peptide derived from human protein. AA range:400-466
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
Vimentin is the most common member of intermediate filament protein family. It is a main component of cytoskeleton structure. Its main function is to maintain cell integrity and skeleton stability. It is expressed in mesenchymal cells and mesoderm derived cells. Vimentin is a relatively specific marker of mesenchymal cells and their derived tumors.
Safety & Handling
This antibody is validated for Immunohistochemistry, Western Blot, Immunofluorescence, ELISA. Recommended dilutions: IHC 1:200-1000;WB 1:500-2000;IF 1:100-500;ELISA 1:1000-5000
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone ABT019.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
