| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | YAP1;YAP65;Yorkie homolog;65 kDa Yes-associated protein;YAP65 |
| Antigen | |
| Cellular Localization | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Formulation | |
| Gene ID | |
| Host | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142 |
| Isotype | |
| Molecular Weight | |
| Product Type | |
| Reactivity | |
| Storage | |
| Target |
Product Overview
U-Blot® YAP (Phospho Ser127) Rabbit Polyclonal Antibody is a rabbit polyclonal antibody from UcallM Biosciences Inc, raised against YAP. Recognizes Phospho. It has been validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA and reacts with Human, Mouse, Rat, Monkey.
Validated Applications
| Target | YAP |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Monkey |
| Applications | Immunofluorescence, Western Blot, Immunohistochemistry, ELISA |
| Purification | Affinity Purified |
| Specificity | Phospho-YAP (S127) Polyclonal Antibody detects endogenous levels of YAP protein only when phosphorylated at S127. |
| Molecular Weight | Observed: 65 kDa |
| UniProt / Swiss-Prot | P46937 (Human) | P46938 (Mouse) | Q2EJA0 (Rat) |
Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;Not yet tested in other applications
Immunogen / Antigen
The antiserum was produced against synthesized peptide derived from human YAP around the phosphorylation site of Ser127. AA range:93-142
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat, Monkey. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
Safety & Handling
This antibody is validated for Immunofluorescence, Western Blot, Immunohistochemistry, ELISA. Recommended dilutions: IF 1:50-200;WB 1:500-1:2000;IHC 1:100-1:300;ELISA 1:40000;Not yet tested in other applications
Confirmed reactivity: Human, Mouse, Rat, Monkey. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
