| Field | Specification |
|---|---|
| Target | |
| Antigen | |
| Alternative names | Yorkie homolog;65 kDa Yes-associated protein;YAP65 |
| Gene ID | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Conjugate | |
| Molecular weight | |
| Purification | |
| Cellular localization | |
| Formulation | |
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| Catalog no. (Mfr.) | |
| Main SKU |
Product Overview
U-Blot® YAP (Phospho Ser397) Rabbit Monoclonal Antibody is a rabbit monoclonal antibody from UcallM Biosciences Inc, raised against YAP. Recognizes Phospho (Ser397). It has been validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA and reacts with Human, Mouse, Rat.
Validated Applications
| Target | YAP |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | PT1357R |
| Isotype | IgG, Kappa |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot, Immunofluorescence, Immunoprecipitation, ELISA |
| Purification | Protein A |
| Specificity | YAP (Phospho Ser397) Antibody detects endogenous levels of YAP protein only when phosphorylated at Ser397.The name of modified sites may be influenced by many factors, such as species (the modified site was not originally found in human samples) and the change of protein sequence (the previous protein sequence is incomplete, and the protein sequence may be prolonged with the development of protein sequencing technology). When naming, we will use the "numbers" in historical reference to keep the sites consistent with the reports. The antibody binds to the following modification sequence (lowercase letters are modification sites):DEsTD |
| Molecular Weight | Calculated: 54 kDa | Observed: 65 kDa |
| UniProt / Swiss-Prot | P46937 (Human) | P46938 (Mouse) | Q2EJA0 (Rat) |
Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Immunogen / Antigen
Transcriptional coactivator YAP1
Reactivity Notes
Confirmed cross-reactivity: Human, Mouse, Rat. Confirm suitability for untested species before use.
Scientific Background
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]
Safety & Handling
This antibody is validated for Western Blot, Immunofluorescence, Immunoprecipitation, ELISA. Recommended dilutions: WB 1:2000-1:10000;IF 1:200-1:1000;ELISA 1:5000-1:20000;IP 1:50-1:200
Confirmed reactivity: Human, Mouse, Rat. Other species should be validated by the user.
Conjugate: Unconjugated.
Store as follows: -15°C to -25°C/1 year(Do not lower than -25°C)
Clone PT1357R.
Customization & Add-ons: Can't find exactly what you need? Contact us for custom formats, conjugations, bulk quantities, or related products.
