UBE2N Antibody, HRP conjugated

Overview

This polyclonal anti-UBE2N antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect UBE2N in Human for epigenetics and nuclear signaling research.

Key elements and design rationale

• Immunogen: Recombinant Human Ubiquitin-conjugating enzyme E2 N protein (20-138AA) — determines epitope; confirm sample prep compatibility.
• Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
• Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
• Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
• Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
• HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.

Biological background

UBE2N (also: Bendless like ubiquitin conjugating enzyme, Bendless-like ubiquitin-conjugating enzyme, BLU, EC 6.3.2.19, Epididymis secretory protein Li 71, HEL-S-71, Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds, MGC131857, MGC8489, UBC 13, Ubc13, UbCH ben, UbcH-ben, UbcH13, UBCHBEN, Ube 2N, Ube2n, UBE2N_HUMAN, Ubiquitin carrier protein N, Ubiquitin conjugating enzyme E2 N, Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13), Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast), Ubiquitin conjugating enzyme E2N, Ubiquitin protein ligase N, Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Yeast UBC13 homolog) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current UBE2N annotation relevant to epigenetics and nuclear signaling.

Common research applications

• ELISA: Quantify soluble target; ensure samples within the linear detection range.

Notes for experimental interpretation

• Isotype controls: IgG from Rabbit at equivalent concentration.
• Cross-reactivity: Polyclonal: validate with KO/KD controls.
• Matrix effects: Verify dilution linearity in your sample matrix.
• Reactivity: Confirmed for Human; extrapolation requires validation.

This product is a polyclonal antibody purified from rabbit antiserum.

• Datasheet (12782012)

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.