| Field | Specification |
|---|---|
| Target | |
| Alternative names | ANCR; Angelman syndrome; AS; CTCL tumor antigen se37 2; CTCL tumor antigen se37-2; E6 AP; E6AP; E6AP ubiquitin protein ligase; E6AP ubiquitin-protein ligase; EPVE6AP; FLJ26981; HECT-type ubiquitin transferase E3A; HPVE6A; Human papilloma virus E6 associated protein Angelman syndrome; Human papilloma virus E6 associated protein; Human papillomavirus E6-associated protein; NY REN 54 antigen; Oncogenic protein associated protein E6 AP; Oncogenic protein associated protein E6AP; Oncogenic protein-associated protein E6-AP; Renal carcinoma antigen NY REN 54; Renal carcinoma antigen NY-REN-54; UBE 3A; Ube3a; UBE3A protein; UBE3A_HUMAN; Ubiquitin protein ligase E3A; Ubiquitin-protein ligase E3A |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Ubiquitin-protein ligase E3A protein (83-234AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-UBE3A antibody raised in Rabbit, conjugated with FITC. Designed to detect UBE3A in Human for epigenetics and nuclear signaling research.
Key elements and design rationale
- Immunogen: Recombinant Human Ubiquitin-protein ligase E3A protein (83-234AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- FITC: Direct fluorescence (Ex490/Em525); protect from light; use antifade.
Biological background
UBE3A (also: ANCR, Angelman syndrome, AS, CTCL tumor antigen se37 2, CTCL tumor antigen se37-2, E6 AP, E6AP, E6AP ubiquitin protein ligase, E6AP ubiquitin-protein ligase, EPVE6AP, FLJ26981, HECT-type ubiquitin transferase E3A, HPVE6A, Human papilloma virus E6 associated protein Angelman syndrome, Human papilloma virus E6 associated protein, Human papillomavirus E6-associated protein, NY REN 54 antigen, Oncogenic protein associated protein E6 AP, Oncogenic protein associated protein E6AP, Oncogenic protein-associated protein E6-AP, Renal carcinoma antigen NY REN 54, Renal carcinoma antigen NY-REN-54, UBE 3A, Ube3a, UBE3A protein, UBE3A_HUMAN, Ubiquitin protein ligase E3A, Ubiquitin-protein ligase E3A) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current UBE3A annotation relevant to epigenetics and nuclear signaling.
Common research applications
- Refer to product documentation for confirmed application data.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
UBE3A is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects UBE3A in immunoassay applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Application data not specified. Consult datasheet or contact BioHippo support.
FITC (Ex490/Em525): direct fluorescence; protect from light; use antifade for sections.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing UBE3A; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
