| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
Recombinant TEV protease (rTEV Protease) is a recombinant protease modified and purified by genetic engineering, which not only maintains the functional activity of natural TEV Protease, but also shows stronger stability and specificity over a broad temperature range. rTEV Protease is a commonly tool enzyme for cutting affinity labels on fusion protein. It is specific to the seven amino acid sequence EXXYXQ↓(G/S), and the cutting site is between glutamine and glycine/serine. The commonly used seven amino acid sequence is Glu-Asn-Leu-Tyr-Phe-Gln↓-Gly. rTEV Protease is optimally active at pH 7.0, 30°C, but is active over a wide range of pH 6.0-8.5 and temperatures 4-30°C (see Table 1), allowing the choice of reaction conditions to be flexible depending on the target protein. In addition, the 6×His label of N-terminal can also be used to remove rTEV Protease by Ni-NTA resin to purify the target protein.
The product has been optimized on the basis of 20403ES, and compared with 20403ES, it has higher purity, stronger activity and lower E.coli gDNA residue, which is suitable for application with higher requirements for enzyme purity, activity and residue.
Features
High cleavage efficiency: The protein tag is removed more thoroughly.
Low residual host gDNA: Effectively reduce the risk of introducing exogenous substance residues.
Applications
Removal of fusion tags
Specifications
|
Unit Definition |
The amount of enzyme required to cut >85% of the 3 μg substrate in a 1×rTEV Buffer (50 mM Tris, pH 8.0, 0.1 mM EDTA, 1 mM DTT) at 30oC for 1 h was defined as a unit of activity |
|
Protein purity |
≥95% |
|
Source |
Expressed in E. coli |
Glycerol Content |
Contains Glycerol |
Components
|
Components No. |
Name |
20427ES80 |
20427ES10 |
20427ES50 |
|
20427-A |
rTEV Protease (5 U/μL) |
200 μL |
2×1 mL |
10 mL |
|
20427-B |
rTEV Buffer (10×) |
600 μL |
6 mL |
30 mL |
Shipping and Storage
rTEV Protease, stored at -25~-15oC for 6 months; Store at -85~-65oC for 1 year.
rTEV Buffer, stored at -25~-15oC for 1 year.
Note: It is recommended to store each component separately when receiving the goods or using it for the first time to avoid repeated freezing and thawing.
Figures
Figure 1. Verification of Cleavage Activity of UCF.METM rTEV Protease
Note: Using a fusion protein (3 μg) containing the UCF.METM rTEV Protease cleavage site as the substrate, UCF.METM rTEV Protease was added at 10, 5, and 3 U, respectively. The reaction was carried out at 30 oC for 1 hour, and the cleavage effect was detected by agarose gel electrophoresis. The results showed that when the amount of UCF.METM rTEV Protease was more than 3 U, it could cleave the substrate efficiently with a cleavage efficiency of >90%.
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Removal of fusion tags. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U/mg) is defined as the amount of protease activity per milligram of enzyme protein under standardized assay conditions (substrate concentration, pH, temperature, and incubation time).
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Most serine proteases (e.g., Proteinase K) are inhibited by PMSF (1 mM) and heat-inactivated at 95°C for 10 min. Proteinase K retains activity across a broad pH range (4–12) and tolerates SDS, urea, and other denaturants, making it particularly effective for nucleic acid extraction from challenging samples including FFPE tissue.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
