| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
T4 Polynucleotide Kinase is a 5'-hydroxyl kinase for polyribonucleotides, capable of catalyzing the transfer of the γ-phosphate group from ATP to the 5'-hydroxyl terminus of oligonucleotide chains (double-stranded or single-stranded DNA or RNA) and to 3'-monophosphate nucleotides. This reaction is reversible. T4 Polynucleotide Kinase also possesses 3'-phosphatase activity, hydrolyzing 3'-phosphate groups from the 3'-phosphate terminus of oligonucleotides, deoxy-3'-monophosphate nucleotides, and deoxy-3'-diphosphate nucleotides. In the presence of ADP, T4 PNK exhibits 5'-phosphatase activity, catalyzing the exchange of 5'-phosphate groups between 5'-P-oligo/polyribonucleotides and ATP. It is suitable for DNA library construction and probe labeling. Compared to T4 Polynucleotide Kinase (Cat#12902ES), UCF.METMT4 Polynucleotide Kinase has lower host residue, making it suitable for applications that require stricter background control, such as pathogen detection.
Applications
Phosphorylation of the 5' terminus of DNA or RNA for ligation reactions.
End labeling of DNA or RNA for probe preparation and DNA sequencing.
Phosphorylation of the 5' terminus of mononucleotides that already have a 3'-phosphate group to prepare pNp substrates for addition to the 3' end of DNA or RNA.
Labeling of the 5' terminus of oligonucleotides that have a 3'-phosphate group.
Components
|
Components No. |
Name |
14503ES76 |
14503ES86 |
14503ES92 |
|
14503-A |
UCF.METM T4 polynucleotide Kinase (10 U/μL) |
250 µL |
1 mL |
|
|
14503-B |
10×T4 PNK Buffer* |
1 mL |
1 mL |
2×1 mL |
Shipping and Storage
This product should be stored at -25 ~ -15℃ for 1 year.
Figures
Figure1. Monomer Phosphorylation Test
Note: A 300 bp monomer ligation test was performed using different batches of UCF.METM T4 Polynucleotide Kinase (10 U/μL) at 5 and 10 U, compared with Brand A. The gel image results showed that Yeasen's product outperformed Brand A in phosphorylation.
Glycerol Content: Contains Glycerol
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
Phosphorylation of the 5' terminus of DNA or RNA for ligation reactions. End labeling of DNA or RNA for probe preparation and DNA sequencing. Phosphorylation of the 5' terminus of mononucleotides that already have a 3'-phosphate group to prepare pNp substrates for addition to the 3' end of DNA or RNA. Labeling of the 5' terminus of oligonucleotides that have a 3'-phosphate group.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the phosphorylation or dephosphorylation of 1 nmol of substrate per minute at 37°C under standard buffer conditions.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of a γ-phosphate from ATP to the 5′-hydroxyl terminus of DNA or RNA, enabling ligation of blunt-end or cohesive-end fragments lacking 5′ phosphates. Shrimp Alkaline Phosphatase (SAP) removes 5′ and 3′ phosphate groups from DNA, RNA, and dNTPs, and is heat-inactivated at 65°C for 15 min—eliminating the need for column cleanup prior to downstream reactions.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
