| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
UCF.ME Uracil DNA Glycosylase derived from psychrophilic marine bacteria, it is an ultra-low residual heat-labile UDG enzyme known as UCF.METM. This product can prevent the residual activity of conventional UDG enzymes after inactivation from degrading dU-containing amplification products at room temperature. It also prevents background bacteria present in molecular enzymes from causing false positives in PCR results. Therefore, the introduction of UCF.METM ultra-low residual thermolabile UDG enzyme is crucial for accurately identifying infectious pathogens and assisting in the clinical diagnosis of infections and infectious diseases.
Features
Ultra-low residual of UCF.METM—E. coli genomic DNA residue <0.1 copies/ U;
Low nuclease residue—No residual exonuclease, nicking enzyme, or RNase;
Strong digestion capability—0.05 U/T can digest 105 copies/T dU-DNA products;
Good thermolability—Complete inactivation under any condition of 50℃ for 10 minutes, 55℃ for 5 minutes, or 95℃ for 5 minutes;
Compatible with RT-qPCR reaction systems—No impact on the detection system even at high input levels (2U/20μL reaction system).
Specifications
Components
|
Name |
14466ES60 100 U |
14466ES76 500 U |
14466ES96 10,000 U |
|
UCF.METM Uracil DNA Glycosylase (UDG/UNG), heat-labile, 1 U/μL |
100 μL |
500 μL |
10 mL |
Storage
The product should be stored at -25~-15°C for 2 years.
Figures
Figure 1. 14466ES protein purity (SDS-PAGE) detection results. Protein Purity≥95%
Figure 2. 14466ES Nuclease residue test results. No Residual Nucleases, No Residual Exonucleases, Nicking Enzymes, or RNases.
Figure 3. 14466ES E.coli genomic DNA residue test results. E.coli genomic DNA residue< 0.1 copies/ U, the residue levels were significantly lower than the conventional UDG.
Figure 4. Strong digestive capability. 0.05 U/T is sufficient to digest 10^5 copies/T of dU-DNA products. 14466ES was used to prepare qPCR mix, while the control group of qPCR mix without UDG enzyme could completely digest 105 copies/T dU-DNA products.
Figure 5. After 50℃, 10min; 55℃, 5min; 55℃, 10min; 95℃, 5min heat inactivation treatment, 14466ES lost digestive ability.
Figure 6. Compatible with the RT-qPCR reaction system, with no impact on the detection system even at high input levels (2U/T).
Store this enzyme at -20°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Dry Ice and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
This enzyme is validated for use in PCR & Amplification, Molecular Diagnostics applications. It is suitable for use in both standard laboratory research settings and, where applicable, optimized reaction workflows requiring high specificity, sensitivity, or throughput.
One unit (U) is defined as the amount of enzyme that catalyzes the release of 1 nmol of uracil from a defined uracil-containing single-stranded DNA substrate in 30 min at 37°C.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Uracil-DNA Glycosylase (UDG/UNG) cleaves the N-glycosidic bond between uracil bases and the sugar-phosphate backbone of DNA containing deoxyuridine (dU). In carryover prevention protocols, dUTP is substituted for dTTP in PCR master mixes. Any carryover amplicon from previous reactions contains dU and is degraded by UDG treatment before PCR cycling, preventing false-positive results. The UDG is inactivated during the initial denaturation step, allowing normal amplification of native dT-containing template DNA.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
