| Field | Specification |
|---|---|
| Target | |
| Alternative names | Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1)|Neuron cytoplasmic protein 9.5|PGP 9.5 (PGP9.5)|Ubiquitin thioesterase L1|UCHL1 |
| Gene ID | |
| UniProt # | |
| Host | |
| Clone | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | ubiquitin carboxyl-terminal esterase L1(ubiquitin thiolesterase) |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Form | Liquid |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
Product Overview
This monoclonal antibody (clone 1H7), raised in Mouse, recognizes UCHL1. It is validated for ELISA, Western Blot, Immunohistochemistry, Immunofluorescence.
Validated Applications
| Applications | ELISA, Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:5000-1:50000 |
| IF | 1:20-1:200 |
| IHC | 1:20-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
ubiquitin carboxyl-terminal esterase L1(ubiquitin thiolesterase)
Specifications
| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Clone | 1H7 |
| Isotype | IgG1 |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | ubiquitin carboxyl-terminal esterase L1(ubiquitin thiolesterase) |
| Purification | Protein A+G Purification |
| Purity | ≥95% as determined by SDS-PAGE |
| Form | Liquid |
| Target MW | 26 kDa |
| UniProt # | P09936 |
| Gene ID | 7345 |
| Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.) |
| Alternative Names | Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1)|Neuron cytoplasmic protein 9.5|PGP 9.5 (PGP9.5)|Ubiquitin thioesterase L1|UCHL1 |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat, Pig. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for UCHL1 research from Fine Test, or contact us for alternative formats.
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