| Field | Specification |
|---|---|
| Mfr No | |
| Detection Range | |
| Product Type | |
| Sample Type(s) | cell culture supernatant, serum, plasma |
| Species | |
| Storage | |
| Target |
Assay Principle
The Tribio® Ultra-sensitive Human IL-33 Fast ELISA is a solid phase ELISA designed to measure human IL-33 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect a concentration as low as 0.5pg/mL, and finish in 2 hours, with no need for 5-6 hours (Fig. 1). The detection range is from 0.5 to 2048 pg/mL. The levels of human IL-33 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human IL-33 protein.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Related Products Human IL-1ß ELISA (TBS3219)
Ultra-sensitive Human IL-33 ELISA Kit is a Solid-phase ELISA for the quantitative determination of Ultra-sensitive IL-33. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Ultra-sensitive IL-33 |
|---|---|
| Species | Human |
| Sample Type | cell culture supernatant, serum, plasma |
| Test Principle | Solid-phase ELISA |
| Detection Range | 0.5–2048 pg/mL |
| Standard | Recombinant Human Ultra-sensitive IL-33 |
| Storage | Store the unopened kit at 2-8°C. | Components: Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8°C.; Aliquot and store at -20°C for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.; May be stored for up to 3 months at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This kit is validated for cell culture supernatant, serum, plasma. Other matrices may require additional in-lab validation.
This assay has a detection range of 0.5–2048 pg/mL. Samples reading outside the range should be diluted or concentrated.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
