| Field | Specification |
|---|---|
| Mfr No | |
| Product Type | |
| Shipping | |
| Source | Recombinant (E. coli) |
| Storage |
UDG (uracil DNA glycosylase) can catalyze the hydrolysis of the N-glycosidic link between the uracil base and the sugar-phosphate backbone in ssDNA and dsDNA. It can easily control aerosol pollution and is suitable for common molecular biology systems such as PCR, qPCR, RT-qPCR and LAMP. Compared to regular UDG, Heat-Labile UDG avoids the potential residual activity that regular UDG may have after inactivation, which could degrade dU-containing amplification products at room temperature.
Applications
1. Remove aerosol contamination from PCR products containing dU.
2. Remove single-stranded or double-stranded DNA uracil bases.
Specifications
|
Unit Definition |
The amount of enzyme required to completely degrade 1 μg of dsDNA containing uracil within 30 minutes at 25°C is defined as 1 activity unit (U). |
|
Deactivation method |
94℃, 2-5 minutes. |
Components
|
No. |
Name |
10707ES60 (100 U) |
10707ES76 (500 U) |
10707ES90 (5 KU) |
10707ES92 (10 KU) |
|
10707 |
Uracil DNA Glycosylase (UDG, Glyceol-free), (1 U/μL) |
100 μL |
500 μL |
5 mL |
10 mL |
Shipping and Storage
Ice pack transport. Store at 4°C, valid for 6 months.
Precautions
- For your safety and health, please wear a lab coat and disposable gloves.
- Thermosensitive UDG is active in most PCR reaction buffers.
Store this enzyme at 2~8°C and avoid repeated freeze-thaw cycles to preserve catalytic activity. The product is shipped Gel Pack and remains stable for up to one year from the date of manufacture when stored under recommended conditions. Aliquoting the stock solution into single-use volumes is recommended for enzymes used infrequently to minimize thermal cycling of the bulk stock.
1. Remove aerosol contamination from PCR products containing dU. 2. Remove single-stranded or double-stranded DNA uracil bases.. Always verify compatibility with your specific template, buffer, and downstream workflow.
One unit (U) is defined as the amount of enzyme that catalyzes the release of 1 nmol of uracil from a defined uracil-containing single-stranded DNA substrate in 30 min at 37°C.
This enzyme is produced as Recombinant (E. coli) and supplied as a Research Use Only (RUO) reagent. The formulation is glycerol-free, making it compatible with lyophilization, high-throughput liquid handling systems, and direct integration into IVD master mix formulations. Each lot is subjected to activity assay, purity assessment by SDS-PAGE, and functional validation prior to release. A Certificate of Analysis (CoA) and Safety Data Sheet (SDS) are available on request.
Uracil-DNA Glycosylase (UDG/UNG) cleaves the N-glycosidic bond between uracil bases and the sugar-phosphate backbone of DNA containing deoxyuridine (dU). In carryover prevention protocols, dUTP is substituted for dTTP in PCR master mixes. Any carryover amplicon from previous reactions contains dU and is degraded by UDG treatment before PCR cycling, preventing false-positive results. The UDG is inactivated during the initial denaturation step, allowing normal amplification of native dT-containing template DNA.
Yeasen Biotechnology supports custom enzyme solutions across multiple service lines — from GMP-grade bulk supply to directed enzyme engineering. Contact BioHippo to discuss requirements and initiate a project inquiry.
▶ GMP-Grade & Bulk Supply
Select Yeasen enzymes are available in GMP grade, manufactured in an ISO 13485-certified UCF.ME™ ultra-clean molecular enzyme facility with FDA Drug Master File (DMF) support.
- GMP-grade release testing and CoA documentation
- ISO 13485-certified production facility
- Scalable from milligram to multi-gram quantities
- Consistent lot-to-lot activity specifications
▶ Glycerol-Free & Custom Formulation
Glycerol-free enzyme formats are available for applications requiring lyophilization compatibility, liquid handling automation, or direct IVD master mix integration.
- Glycerol-free liquid format (standard and custom buffers)
- Lyophilization-ready enzyme preparation
- Custom reaction buffer optimization for specific assay conditions
- Compatible with freeze-drying workflows for point-of-care formats
▶ Molecular IVD RDC Service
Yeasen's Research and Development Contracting (RDC) team delivers end-to-end solutions for molecular diagnostic product development, covering enzyme selection through clinical validation support.
- Enzyme selection and performance matching
- Primer/probe design and reaction buffer optimization
- Sensitivity, specificity, and precision validation studies
- Stability studies and SNP evaluation
- Instrument platform compatibility assessment
▶ ZymeEditor™ Enzyme Engineering
Yeasen's proprietary ZymeEditor™ directed evolution and rational design platform enables the development of custom enzyme variants with tailored performance characteristics not available in off-the-shelf products.
- Directed evolution for enhanced thermostability, processivity, or fidelity
- Rational design for altered substrate specificity or cofactor requirements
- Library screening from Yeasen's proprietary enzyme variant collection
- Scale-up to commercial quantities upon candidate confirmation
ⓘ Customization services are fulfilled by Yeasen Biotechnology. Lead times and minimum order quantities vary by service type. Contact BioHippo for project scoping and pricing.
