| Field | Specification |
|---|---|
| Target | |
| Alternative names | AAH67261; Deubiquitinating enzyme 48; DKFZp762M1713; EC 3.1.2.15; Kiaa4202; MGC132556; MGC14879; RAP1GA1; Synaptic ubiquitin specific protease; synUSP; Ubiquitin carboxyl terminal hydrolase 48; Ubiquitin carboxyl-terminal hydrolase 48; Ubiquitin specific peptidase 48; Ubiquitin specific processing protease 48; Ubiquitin specific protease 31; Ubiquitin specific protease 48; Ubiquitin thioesterase 48; Ubiquitin thiolesterase 48; Ubiquitin-specific-processing protease 48; UBP48_HUMAN; USP31; USP48 |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Ubiquitin carboxyl-terminal hydrolase 48 protein (358-508AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-USP48 antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect USP48 in Human for cell biology research.
Key elements and design rationale
- Immunogen: Recombinant Human Ubiquitin carboxyl-terminal hydrolase 48 protein (358-508AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- Biotin: Streptavidin/avidin amplification; block endogenous biotin in biotin-rich samples.
Biological background
USP48 (also: AAH67261, Deubiquitinating enzyme 48, DKFZp762M1713, EC 3.1.2.15, Kiaa4202, MGC132556, MGC14879, RAP1GA1, Synaptic ubiquitin specific protease, synUSP, Ubiquitin carboxyl terminal hydrolase 48, Ubiquitin carboxyl-terminal hydrolase 48, Ubiquitin specific peptidase 48, Ubiquitin specific processing protease 48, Ubiquitin specific protease 31, Ubiquitin specific protease 48, Ubiquitin thioesterase 48, Ubiquitin thiolesterase 48, Ubiquitin-specific-processing protease 48, UBP48_HUMAN, USP31, USP48) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current USP48 annotation relevant to cell biology.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
USP48 is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects USP48 in ELISA applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
Biotin: streptavidin/avidin amplification. Block endogenous biotin in biotin-rich samples.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing USP48; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
