| Field | Specification |
|---|---|
| Target | |
| Alternative names | Deubiquitinating enzyme FAF X; Deubiquitinating enzyme FAF-X; DFFRX; Drosophila fat facets related X linked; FAF; Fafl; Fam; Fat facets homolog; Fat facets in mammals; Fat facets protein related X linked; Fat facets protein related, X-linked; Fat facets protein-related; hFAM; MRX99; Probable ubiquitin carboxyl terminal hydrolase FAF X; Probable ubiquitin carboxyl-terminal hydrolase FAF-X; Ubiquitin carboxyl-terminal hydrolase FAM; Ubiquitin specific peptidase 9 X linked; Ubiquitin specific peptidase 9, X-linked; Ubiquitin specific processing protease FAF X; Ubiquitin specific protease 9 X chromosome; Ubiquitin thioesterase FAF X; Ubiquitin thiolesterase FAF X; Ubiquitin thiolesterase FAF-X; Ubiquitin-specific protease 9; Ubiquitin-specific-processing protease FAF-X; USP9 (gene name); Usp9x; USP9X_HUMAN; Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila); X chromosome; X-linked |
| UniProt # | |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Recombinant Human Probable ubiquitin carboxyl-terminal hydrolase FAF-X protein (2360-2484AA) |
| Conjugate | |
| Source | This product is a polyclonal antibody purified from rabbit antiserum. |
| Purification | |
| Form | Liquid |
| Storage | |
| Storage buffer | |
| Catalog no. (Mfr.) | |
| Main SKU |
Overview
This polyclonal anti-USP9X antibody raised in Rabbit, conjugated with HRP, tested in ELISA. Designed to detect USP9X in Human for cell biology research.
Key elements and design rationale
- Immunogen: Recombinant Human Probable ubiquitin carboxyl-terminal hydrolase FAF-X protein (2360-2484AA) — determines epitope; confirm sample prep compatibility.
- Host (Rabbit): Requires anti-rabbit-IgG secondary matching isotype IgG.
- Polyclonal: Multiple epitopes = robust signal; lot-to-lot variability — include controls.
- Isotype (IgG): Match secondary, Protein A/G, and isotype controls to IgG.
- Purification (Protein G purification): Enriches Ig classes; reduces non-specific background.
- HRP: Direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
Biological background
USP9X (also: Deubiquitinating enzyme FAF X, Deubiquitinating enzyme FAF-X, DFFRX, Drosophila fat facets related X linked, FAF, Fafl, Fam, Fat facets homolog, Fat facets in mammals, Fat facets protein related X linked, Fat facets protein related, X-linked, Fat facets protein-related, hFAM, MRX99, Probable ubiquitin carboxyl terminal hydrolase FAF X, Probable ubiquitin carboxyl-terminal hydrolase FAF-X, Ubiquitin carboxyl-terminal hydrolase FAM, Ubiquitin specific peptidase 9 X linked, Ubiquitin specific peptidase 9, X-linked, Ubiquitin specific processing protease FAF X, Ubiquitin specific protease 9 X chromosome, Ubiquitin thioesterase FAF X, Ubiquitin thiolesterase FAF X, Ubiquitin thiolesterase FAF-X, Ubiquitin-specific protease 9, Ubiquitin-specific-processing protease FAF-X, USP9 (gene name), Usp9x, USP9X_HUMAN, Uubiquitin specific protease 9, X chromosome (fat facets like Drosophila), X chromosome, X-linked) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current USP9X annotation relevant to cell biology.
Common research applications
- ELISA: Quantify soluble target; ensure samples within the linear detection range.
Notes for experimental interpretation
- Isotype controls: IgG from Rabbit at equivalent concentration.
- Cross-reactivity: Polyclonal: validate with KO/KD controls.
- Matrix effects: Verify dilution linearity in your sample matrix.
- Reactivity: Confirmed for Human; extrapolation requires validation.
This product is a polyclonal antibody purified from rabbit antiserum.
USP9X is a protein studied in Human biology. This polyclonal antibody raised in Rabbit detects USP9X in ELISA applications, IgG isotype.
Reported reactive against Human. Immunogen from Human. Cross-reactivity with untested species requires empirical confirmation.
Reported for ELISA. Optimize dilution/blocking per application. Consult datasheet for working concentrations.
HRP: direct detection (TMB/ECL); quench endogenous peroxidase in tissue assays.
(1) Isotype control: IgG from Rabbit; (2) Positive control: lysate/cell line expressing USP9X; (3) Negative control: siRNA KD or KO; (4) Dilution linearity in your sample matrix.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
