| Field | Specification |
|---|---|
| Target | |
| Alternative names | Vasodilator-stimulated phosphoprotein; VASP |
| Host | |
| Clonality | |
| Isotype | |
| Reactivity | |
| Applications | |
| Immunogen | Synthetic peptide, corresponding to amino acids 200-250 of Human VASP. |
| Conjugate | |
| Molecular weight | |
| Purity | |
| Purification | |
| Formulation | |
| Storage | |
| Catalog no. (Mfr.) | |
| Main SKU |
Scientific Background
VASP (vasodilator-stimulated phosphoprotein), is involved in the maintenance of cytoarchitecture by interacting with actin-like filaments. VASP shares a limited degree of homology with the amino terminus of WASP, which is frequently mutated in WAS patients. An established substrate of cAMP and cGMP dependent kinases, VASP is phosphorylated on a regulatory serine residue 157 and localizes to focal adhesions, microfilaments and highly active regions of the plasma membrane. VASP is a protein of between 46 and 50 kDa that is highly expressed in human platelets and, like WASP, may play a role in cytoskeletal organization.
Product Overview
This polyclonal antibody, raised in Rabbit, recognizes VASP. VASP (A232) polyclonal antibody detects endogenous levels of VASP protein.
Validated Applications
| Applications | Western Blot, Immunohistochemistry, Immunofluorescence |
|---|---|
| WB | 1:500-1:1000 |
| IHC | 1:50-1:200 |
| IF | 1:50-1:200 |
Optimal dilutions should be determined by the end user for each application and sample type.
Immunogen
Synthetic peptide, corresponding to amino acids 200-250 of Human VASP.
Specifications
| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Immunogen | Synthetic peptide, corresponding to amino acids 200-250 of Human VASP. |
| Purity | > 95% (by SDS-PAGE) |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Formulation | Rabbit IgG, 1 mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2 |
| Target MW | ~ 46,50 kDa |
| Swiss-Prot | P50552 |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
Reactivity Notes
Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls before critical experiments.
Safety & Handling
This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.
Related Products
Explore related antibodies and reagents for VASP research from Bioworld Technology Inc, or contact us for alternative formats.
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