| Field | Specification |
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| Assay Time | |
| Detection Range | |
| Product Type | |
| Sensitivity | |
| Storage | |
| Target |
Assay Principle
The Tribioscience Vero cell HCP Fast ELISA is designed to quantitatively detect Vero cell HCP residues in different biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay flexible, accurate, simple, and time saving. The measurement can be finished in 2 hours, not needing 4-5 hours (Fig. 1). The detection range is from 1 to 200 ng/mL. The levels of Vero HCP samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for Vero host cell protein.
Typical Data This standard curve is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
The Intra-assay CV is 3.79% and the Inter-assay CV is ˂10%.
Vero Cell Host Cell Protein ELISA Kit is a Solid-phase ELISA for the quantitative determination of Vero Cell Host Cell Protein. The microplate is pre-coated with a capture antibody specific to the target. Target present in the sample or standard binds the immobilized antibody, is detected with a biotinylated detection antibody and streptavidin-HRP, and quantified colorimetrically against a standard curve.
Kit Components
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. Refer to the product datasheet for the complete component list and quantities.
Performance Data
| Target | Vero Cell Host Cell Protein |
|---|---|
| Test Principle | Solid-phase ELISA |
| Sensitivity | 1.1 ng/mL |
| Detection Range | 1–200 ng/mL |
| Assay Time | 2 hours |
| Precision | Intra-assay CV 3.79%; Inter-assay CV 10% |
| Specificity | This assay recognizes Vero HCP. |
| Storage | Store the unopened kit at 2-8°C. |
✓ Quality tested for reproducibility and lot-to-lot consistency
Safety & Handling
This assay has a sensitivity of 1.1 ng/mL and a detection range of 1–200 ng/mL. Samples reading outside the range should be diluted or concentrated.
The total assay time is approximately 2 hours from sample addition to plate reading.
The kit provides a pre-coated microplate, target standard for standard-curve generation, detection reagents (detection antibody and enzyme conjugate), wash buffer, substrate, and stop solution. See the datasheet for the full component list and quantities.
A standard microplate reader capable of measuring absorbance at 450 nm is required for this sandwich ELISA.
Customization & Add-ons: Need this ELISA kit tailored to your workflow? We can help with bulk or custom pack sizes, species or analyte variants, matched antibody pairs and standards, alternate plate formats (48- or 96-well, strip-well), and assay development or custom ELISA solutions for targets not in our catalog. Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services — our team will follow up with feasibility details and next steps.
