Vero E6 cell

SKU:BHC11101280
Bulk Pricing Research Validated
Overview
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Vero E6 cell is a cell line. It is commonly used as an in vitro model for 1 research. Growth characteristics: Adherent, Epithelial. Supplied as cryopreserved cells with accompanying batch CoA and quality-control documentation.

Species Chlorocebus sabaeus (Green monkey)
Morphology Epithelial
Growth Properties Adherent
Tissue Normal Kidney
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Catalog no. Size
305008 1 cryovial
Available Options

This cell line is available in the U.S. For non-profit users, please sign and submit the Non-Profit Supply Agreement to orders@biohippo.com before placing an order. For commercial users, please complete the CLEAR Form before ordering, as additional usage fees may apply based on the intended use. For further details, please contact orders@biohippo.com. Products ship after the required agreement is completed; typical delivery is 2–3 business days. Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10^6 cells for adherent lines or 5 × 10^6 cells for suspension lines (refer to the batch CoA for details).

Field Specification
Mfr No 305008
Species Chlorocebus sabaeus (Green monkey)
Vero E6 cells, also known as Vero C1008 or Vero 76 clone E6, are a continuous line of epithelial cells derived from the kidney of the African green monkey, Chlorocebus sabaeus. The Vero clone E6, a subline of Vero cells, is particularly noted for its utility in virology research due to its high susceptibility to a wide range of viruses, including coronaviruses like SARS-CoV and SARS-CoV-2, the Ebola virus, and the Zika virus. The cell line is crucial in the production of vaccines, such as those for Japanese Encephalitis vaccine, due to their capacity for virus culture and isolation. The cells have played a pivotal role in the development of COVID therapeutics, including the testing of the polymerase inhibitor remdesivir. With their ability to support the replication of a variety of viruses, Vero E6 cells facilitate compound screening and the evaluation of antiviral efficacy. Their role in clinical trials extends to the assessment of anti-inflammatory drugs like dexamethasone and the study of gene products like the P-glycoprotein (pgp protein) encoded by the pgp gene. Vero E6 cells lack the interferon-β gene, which partly explains their high susceptibility to viral infections; this deficiency prevents them from mounting an effective innate antiviral response. In summary, Vero E6 cells are a valuable resource in the field of virology and biomedicine, providing a versatile platform for antiviral screening, the study of replication in Vero, and aiding in the quest for understanding retroviral sequences.

SKU:BHC11101280

  • cultureMedium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
  • supplements: Supplement the medium with 10% FBS and 1% NEAA
  • dissociationReagent: Accutase
  • doublingTime: 22 hours
  • subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
  • fluidRenewal: 2 to 3 times per week
  • freezeMedium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
  1. Biological activities (antimicrobial, anti-inflammatory, anti-coronavirus (229E)) and chemical composition of some essential oilsScientific Reports| DOI: 10.1038/s41598-026-48032-1 | PMID: 42031843 | PMC: pmc13109404
  2. The core herpes simplex-1 fusion complex drives cell-to-cell spreading of pathological TaubioRxiv| DOI: 10.64898/2026.01.21.700770 | PMC: bio_rxiv__64898__2026__01__21__700770
  3. Single MVA-SARS-2-ST/N Vaccination Rapidly Protects K18-hACE2 Mice against a Lethal SARS-CoV-2 Challenge InfectionViruses| DOI: 10.3390/v16030417 | PMID: 38543782 | PMC: pmc10974247
  4. UntitlediScience| DOI: 10.1016/j.isci.2024.110354 | PMC: pmc11277385
  5. Tetrazolylidene-Stabilized Gold(I) Complexes: Synthesis and Evaluation of Anticancer Activity In VitroInorganic Chemistry| DOI: 10.1021/acs.inorgchem.6c00049 | PMID: 41985072 | PMC: pmc13126643
  6. Cowpox virus but not Vaccinia virus induces secretion of CXCL1, IL-8 and IL-6 and chemotaxis of monocytes in vitro.Virus research| DOI: 10.1016/j.virusres.2012.11.013 | PMID: 23207068 | PMC: pm23207068
  7. Stabilized recombinant SARS-CoV-2 spike antigen enhances vaccine immunogenicity and protective capacityThe Journal of Clinical Investigation| PMID: 36301637 | PMC: pmc09754005__jci___132___159895___s033
  8. Integrating artificial intelligence-based epitope prediction in a SARS-CoV-2 antibody discovery pipeline: caution is warrantedeBioMedicine| DOI: 10.1016/j.ebiom.2023.104960 | PMID: 38232633 | PMC: pmc10803917__mmc5
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