Vimentin Polyclonal Antibody

SKU:BHA12110737
Overview
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Anti-VIM antibody (Rabbit, polyclonal, IgG, unconjugated). Validated for Western Blot. Reacts with Human, Mouse, Rat. Suitable for routine research immunoassays. Immunogen: Recombinant Protein of Vimentin.
Clonality Polyclonal
Host Rabbit
Reactivity Human, Mouse, Rat
Applications Western Blot
Conjugate Unconjugated
Target MW 53652 Da
UniProt Human: P08670; Mouse: P20152; Rat: P31000
Options selector
Catalog no. Size
BT-AP09527-20UL 20 uL
BT-AP09527-50UL 50 uL
BT-AP09527-100UL 100 uL
Available Options

Select the variant that best fits your experiment. Availability and lead time may vary by option.

  • Options: Size: 20 uL, 50 uL, 100 uL
  • Lead time: options listed in "Availability Content"; other statuses may take longer.
  • Storage: -20°C for 1 year
  • Shipping: Please contact us to confirm shipping conditions for this product.
  • Upon receipt: store at recommended temperature as soon as possible.
  • Sales terms and conditions: Please review prior to ordering.
Field Specification
Target VIM
Alternative names VIM; Vimentin
Gene ID Human: 7431; Mouse: 22352; Rat: 81818
UniProt # Human: P08670; Mouse: P20152; Rat: P31000
Host Rabbit
Clonality
  • Polyclonal
Isotype
  • IgG
Reactivity
  • Human
  • Mouse
  • Rat
Applications
  • Western Blot
Immunogen Recombinant Protein of Vimentin
Conjugate
  • Unconjugated
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Concentration 1 mg/ml
Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Storage -20°C for 1 year
Catalog no. (Mfr.) BT-AP09527
Main SKU BHA12110737
Polyclonal Antibody

Scientific Background

VIM encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin encoded by VIM is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Product Overview

This polyclonal antibody, raised in Rabbit, recognizes VIM. It is validated for Western Blot.

Validated Applications

Applications Western Blot

Optimal dilutions should be determined by the end user for each application and sample type.

Immunogen

Recombinant Protein of Vimentin

Specifications

Host Rabbit
Clonality Polyclonal
Isotype IgG
Conjugate Unconjugated
Reactivity Human, Mouse, Rat
Immunogen Recombinant Protein of Vimentin
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Concentration 1 mg/ml
Formulation PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Target MW 53652 Da
UniProt Accession Human: P08670; Mouse: P20152; Rat: P31000
Gene ID Human: 7431; Mouse: 22352; Rat: 81818
Storage -20°C for 1 year
Lead Time Within one week

Reactivity Notes

Validated reactivity: Human, Mouse, Rat. For other species, epitope conservation may allow cross-reactivity; confirm with appropriate controls.

Safety & Handling

This product is for research use only (RUO) and is not intended for diagnostic or therapeutic use. The formulation contains sodium azide; handle with standard laboratory precautions.

Related Products

Explore related antibodies and reagents for VIM research from Bioassay Technology Laboratory, or contact us for alternative formats.

Q.Which applications is this antibody validated for?
A.Validated for Western Blot. Determine optimal dilutions empirically.
Q.What are the host, clonality, and isotype?
A.Raised in Rabbit as a polyclonal antibody, isotype IgG. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen..
Q.What was the immunogen?
A.Recombinant Protein of Vimentin
Q.Which species does it react with?
A.Human, Mouse, Rat. Validate cross-reactivity for unlisted species with appropriate controls.
Q.How should I store and handle this antibody?
A.-20°C for 1 year Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.. Shipped with cold-chain packaging. RUO - not for diagnostic or therapeutic use.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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